DNN+NeuroSim is an integrated framework to benchmark compute-in-memory (CIM) accelerators for deep neural networks, with hierarchical design options from device-level, to circuit level and up to algorithm level. A python wrapper is developed to interface NeuroSim with a popular machine learning platform: Pytorch, to support flexible network structures. The framework provides automatic algorithm-to-hardware mapping, and evaluates chip-level area, energy efficiency and throughput for training or inference, as well as training/inference accuracy with hardware constraints. Our prior inference version of DNN+NeuroSim framework available at https://github.com/neurosim/DNN_NeuroSim_V1.2 was developed to estimate the impact of reliability in synaptic devices, and analog-to-digital converter (ADC) quantization loss on the accuracy and hardware performance of an inference engine. In this work, we further investigated the impact of the “analog” emerging nonvolatile memory (eNVM)’s nonideal device properties for on-chip training. By introducing the nonlinearity, asymmetry, device-to-device and cycle-to-cycle variation of weight update into the python wrapper, and peripheral circuits for error/weight gradient computation in NeuroSim core, we benchmarked CIM accelerators based on state-of-the-art SRAM and eNVM devices for VGG-8 on CIFAR-10 dataset, revealing the crucial specs of synaptic devices for on-chip training. The latest training version of the DNN+NeuroSim framework is available at https://github.com/neurosim/DNN_NeuroSim_V2.1 .